Table 2. Differential metabolites identified by LC-MS secondary mass spectrometry.
Metabolin | Type | Mass | RT.s | vip | p corr | p value | fold_change |
---|---|---|---|---|---|---|---|
Glutathione | [M + H]+ | 307.1 | 57.5 | 8.4 | −0.91 | 2.74E-06 | −1.03 |
cGMP | [M − H]− | 345.0 | 58.0 | 1.4 | −0.89 | 5.71E-04 | −0.86 |
5-phosphoribosyl-1-diphosphate | [M − H]− | 390.0 | 35.1 | 2.6 | −0.85 | 2.40E-03 | −0.62 |
dTMP | [M − H]− | 322.1 | 70.4 | 2.0 | 0.88 | 8.13E-04 | 0.70 |
Cysteine | [M + H]+ | 121.0 | 59.1 | 2.2 | 0.88 | 1.16E-08 | 0.94 |
Adenine | [M + H]+ | 135.1 | 195.9 | 3.2 | 0.89 | 1.07E-05 | 1.01 |
2-Chloroadenosine | [M + H]+ | 301.1 | 409.6 | 5.5 | 0.81 | 1.58E-06 | 1.07 |
Xanthine | [M + H]+ | 152.0 | 75.5 | 3.0 | 0.90 | 3.31E-06 | 1.10 |
Uridine 5-diphosphoglucuronic acid | [M − H]− | 580.0 | 48.5 | 1.6 | 0.92 | 9.11E-04 | 1.19 |
Xanthosine | [M + H]+ | 284.1 | 171.4 | 1.3 | 0.89 | 5.76E-09 | 1.22 |
Uridine monophosphate (UMP) | [M − H]− | 324.0 | 46.4 | 10.4 | 0.98 | 2.62E-06 | 1.32 |
Adenylosuccinate | [M − H]− | 463.1 | 126.0 | 15.3 | 0.96 | 1.07E-04 | 1.35 |
Type, ionization mode; [M + H]+, positive ion mode; [M − H]−, negative ion mode; Mass, exact molecular weight; RT.s, retention time/second; vip, PLS-DA first principal component variable importance in projection; pvalue, t-test significance; Pcorr, p (corr) value of S-plot correlation; fold_change, logarithm of Per-KD/WT fold change (fold change >0 means WT > Per-KD; fold change <0 means WT < Per-KD). All the metabolites listed here did MS/MS.