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Comparison of the extent of fusion promoted by wt F and L289A-F expressed with different NDV-hPIV3 HN chimeras. Proteins are expressed in BHK cells driven by the vaccinia virus-T7 RNA polymerase expression system. The extent of fusion is quantitated with the content-mixing fusion assay and expressed as the percentage of that obtained with wt HN and wt F. Error bars show the standard deviation from at least four independent determinations.
