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. 2017 Mar 15;139(13):4659–4662. doi: 10.1021/jacs.7b01459

Scheme 1. Design of a Chemical-Inducible Epigenome Remodeling (CiDER) Tool Based on a Split TET2 Enzyme.

Scheme 1

FKBP12/FRB heterodimerization or FKBP-F36 V homodimerization modules are fused with two inactive fragments of a split TET2CD. Upon the addition of rapamycin or AP1903, split TET2CD fragments reassemble into a functional methylcytosine dioxygenase to catalyze the conversion of 5mC into 5hmC and further oxidized species, thus promoting DNA demethylation to remodel the epigenetic landscapes in mammalian cells.