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. 2017 Apr 10;7:46315. doi: 10.1038/srep46315

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Copyright © 2017, The Author(s)

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. (A) The qRT-PCR analysis of EpCAM expression during porcine fibroblast reprogramming by hOSKM for 14 days. (B) Transduction of pTRIP-EpCAM-EGFP and pTRIP-CAGG-EGFP (Ctrl) into PEF, PK-15, and piPS cells, respectively, to determine activation of the EpCAM promoter. Scale bar, 100 μm. (C) Luciferase assay. Reporter pGL3-EpCAM-Pro (Exp) and pGL3-Basic (Ctrl) were transfected into PEF and PK-15 cells, respectively, for 36 h. (D) Luciferase assay of EpCAM promoter activation regulated by human pluripotent factor alone (left), or combination (right) in 293 T cells. Ctrl, cells were transfected with pGL3-EpCAM-Pro and pMXs-EGFP. (E) Luciferase assay of EpCAM promoter activation that was regulated by porcine pluripotent factors. Data are presented as mean ± S.D., *P < 0.05, **P < 0.01, n = 3.