Table 1. Geometrical characteristics of HUAECs.
| Area (μm2) | Perim. (μm) | Major Ax. (μm) | Minor Ax. (μm) | Aspect Ratio | Circular. | Round. | ||
|---|---|---|---|---|---|---|---|---|
| Clone 1 | Control | 6372 ± 275 | 321 ± 7 | 104 ± 2 | 75 ± 2 | 1.40 ± 0.02 | 0.75 ± 0.01 | 0.73 ± 0.01 |
| MSX1 | 4405 ± 114 | 380 ± 6 | 155 ± 2 | 36 ± 1 | 4.46 ± 0.11 | 0.39 ± 0.01 | 0.24 ± 0.01 | |
| Clone 2 | Control | 6187 ± 273 | 309 ± 7 | 109 ± 3 | 71 ± 2 | 1.55 ± 0.04 | 0.80 ± 0.01 | 0.66 ± 0.02 |
| MSX1 | 2077 ± 86 | 298 ± 7 | 123 ± 3 | 21 ± 1 | 6.02 ± 0.21 | 0.30 ± 0.01 | 0.18 ± 0.01 |
Cells from two independent clones were transduced with Cherry- (control) or MSX1-encoding lentiviruses (MSX1). Aspect ratio is the coefficient between the major and the minor axes, circularity:
, and roundness:
. MSX1-overexpressing cells have a higher aspect ratio, and lower circularity and roundness than control cells. Values are displayed as mean ± s.e.m. of the total amount of cells ‘n’ measured; n = 100 for control and MSX1 in clone 1; n = 54, 71 in clone 2 for control and MSX1, respectively.