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. 2017 Apr 10;7:46204. doi: 10.1038/srep46204

Figure 6. LINC00305 promotes AHRR protein expression and nuclear localization to activate NF-κB.

Figure 6

(A) Reporter assay of NF-κB activity in 293 T cells transfected with different combinations of LINC00305, LIMR and AHRR. 293 T cells were transfected with LINC00305, with or without LIMR- and AHRR-expressing vectors, and the pNF-κB-TA-luc and pRL-TK reporters. The luciferase activity (firefly/Renilla) was measured using a Modulus Microplate Multimode Reader. Data are presented as the mean ± sem of 3 independent experiments. *p < 0.05, ns: no significance vs. the indicated group. (B) Real-time RT-PCR analysis of AHR and AHRR expression in THP-1 cells stably expressing LINC00305 or the control vector. Gene expression levels in the control groups were assigned a value of 1.0, and GAPDH was used as an internal control. Data are presented as the mean ± sem of 3 independent experiments. *p < 0.05, ns: no significance vs. the indicated group. (C) Western blotting assay of AHR and AHRR expression in THP-1 cells stably expressing LINC00305 or the control vector. β-Actin and GAPDH were used as the internal controls. (D,E) Immunofluorescence assays of AHRR (D) and AHR (E) localization in THP-1 cells stably expressing LINC00305 or the control vector.