FIG. 2.

SUMO-1 covalently modifies p6. Human 293T cells were transfected with plasmids encoding the proteins indicated above each lane. Cell lysates were analyzed by Western blotting with antibodies against GFP (top), the HA epitope tag (middle), or SUMO-1 (bottom). The positions of migration of size markers (in kilodaltons) are indicated on the left of the panels. The positions of migration of the expressed proteins, as well as of GFPp6 covalently attached to HASUMO-1, are indicated on the right of the blots. Note that the anti-SUMO-1 antibody seems to nonspecifically recognize GFP-p6.