FIG. 7.

Representative oxymetric traces indicating rates of oxygen consumption of sham, vehicle, and cyclosporine A (CsA) (20 mg/kg, intraperitoneally, 15 min post-injury) for (A) non-synaptic and (B) synaptic mitochondria isolated from ipsilateral cortex 24 h after severe controlled cortical impact (CCI). Purified mitochondrial protein (>30 μg) was suspended in respiration buffer (125 mmol/L KCl, 2 mmol/L MgCl₂, 2.5 mmol/L KH₂PO₄, 0.1% BSA, 20 mmol/L HEPES, pH 7.2) in a final volume of 250 μl, and oxygen consumption rates were measured using a Clark-type oxygen electrode in the presence of 5 mmol/L pyruvate and 2.5 mmol/L malate (state II), two boluses of 150 μmol/L ATP (state III), 2 μmol/L oligomycin (state IV), 2 μmol/L FCCP (state VI), and 100 nmol/L rotenone and 10 mmol/L succinate (state VII). Sham, sham; CsA, CCI + CsA; vehicle, CCI + vehicle; ADP, adenosine diphosphate.