FIG. 3.
Ebola virus GP-mediated entry and fusion to human monocyte-derived macrophages. (A) Monocyte-derived macrophages were incubated for 3 h with BlaM-Vpr-containing virions lacking Env or pseudotyped with Ebola virus GP, HIV-1HXB2, or VSV-G. (B) Efficiency of Ebola virus GP-mediated entry and fusion in human monocytes versus macrophages. Human monocytes and macrophages (2 × 106) derived from six donors were incubated with the same concentration of Ebola virus GP pseudotypes for 3 h. Percentages of blue cells represent cells supporting virion entry and fusion. Error bars indicate standard deviations derived from triplicate samples.