FIG. 5.

Effects of actin-disrupting or -stabilizing agents on Ebola virus GP-mediated entry and fusion. (A) Fluorescence labeling of actin filaments in HeLa cells treated with CytB (20 μM), CytD (20 μM), LatA (1 μM), or Jas (1 μM) for 30 min at 37 °C followed by labeling with Texas Red-X phalloidin (CyB, CyD, LatA, and Jas) or anti-β-actin antibody (Jas). (B to E) HeLa and HeLa-CD4 cells were pretreated with the indicated concentrations of CytB (B), CytD (C), LatA (D), or Jas (E) at 37°C for 30 min and incubated with each of the pseudotyped virions in the continued presence of each drug at 37°C for 3 h. Values represent the level of entry and fusion relative to that in untreated controls.