Fig. 2.

C. elegans get tired after acute swim exercise. a The acute swim exercise (M9 buffer) and control (unseeded plate crawl) protocol for C. elegans. b Crawling distance traveled by N2 animals at different time points after a 90 min swim exercise. Each point represents a single animal (n = 49–50 animals). c Crawling distance ratio of exercise to control N2 animals 5 min after a swim exercise of displayed durations (n = 40–50 animals). Unpaired two-tailed Student’s t test was used in (b) and (c) to compare crawling distances of control versus exercise animals. *P < 0.05; ****P < 0.0001. NGM nematode growth medium, ns non-significant