Fig. 5.

Transcriptional changes in C. elegans suggest increased fat metabolism during swim exercise. a Diagram of fat metabolism highlighting the steps that we chose for our expression analysis. C. elegans proteins are shown in red. For simplicity, several metabolic steps are omitted. b Heat map summarizing quantitative polymerase chain reaction results in N2 animals at different time points post-exercise for fat metabolic genes (n = 5 independent trials). Expression data is presented as log₂ fold change of exercise samples relative to control samples in a color gradient from red (downregulation) to dark green (upregulation). White represents no change in expression levels. Paired two-tailed Student’s t tests were used to compare relative expressions of control versus exercise samples at each time point. See Additional file 6 for detailed results for each gene. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001