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. 2017 Apr 1;34(7):1364–1381. doi: 10.1089/neu.2016.4569

FIG. 5.

FIG. 5.

Post-imaging neuroinflammation in the corpus callosum and cortex after r-mTBI. Immunohistochemistry was used to detect microglia/macrophage cells with CD11b and astrocytes with GFAP in mice perfused after the final imaging scan, that is, 6 weeks post-injury or sham procedure. (A and B) Slight astrogliosis and microglia/macrophage activation were evident in r-mTBI mice (B) compared to r-sham mice (A). (C–H) Quantitative analysis shows a significant increase in microglial activation in the corpus callosum of r-mTBI mice relative to sham (C and D). Astrogliosis in the corpus callosum was not significantly increased after r-mTBI (E). In the cortex, the r-mTBI mice had a significant increase in the area of CD11b immunolabeling and in the number of activated microglia (F and G). Astrogliosis in the medial cortex was not significantly increased after r-mTBI (H). Dashed lines outline the corpus callosum. Values are mean ± standard error of the mean; n = 5; scale bar = 50 μm; *p < 0.05. DAPI, 4′,6-diamidino-2-phenylindole; GFAP, glial fibrillary acidic protein; r-mTBI, repetitive mild traumatic brain injury; r-sham, repetitive sham.