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. 2017 Jan 31;27(4):483–504. doi: 10.1038/cr.2017.18

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Copyright © 2017 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

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Precise labeling of telomeres with thioredoxin-fused TALEs (TTALEs). (A) Schematic illustration of TALEs fused with various solubility-enhancing peptides (X) to label telomeres. (B) Co-localization analysis of telomeric FISH (red) and Flag-TALE^telo (green) fused with the indicated peptides in HeLa cells. Engineered TALE^telo was visualized by immunostaining with anti-Flag antibody. Representative images using various fusion partners show that precise co-localization with telomeric FISH signals was obtained only using the thioredoxin-fused TALE^telo (TTALE^telo). Dashed lines indicate the nuclear boundary. Scale bars, 5 μm. (C) Histograms showing numbers of telomeric FISH- and peptide-fused TALE^telo(19 bp)-positive dots in nuclei of HeLa cells. n = 50 nuclei per group.