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. 2017 Jan 31;27(4):483–504. doi: 10.1038/cr.2017.18

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Copyright © 2017 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

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Precise labeling of centromeres with TTALEs. (A) Schematic illustration of TALEs fused with various solubility-enhancing peptides (X) to label centromeres. (B) Co-localization analysis of centromeric FISH (red) and Flag-TALE^centro (green) fused with the indicated peptides in HeLa cells. Engineered TALE^centro was visualized by immunostaining with anti-Flag antibody. Representative images show precise co-localization of centromeric FISH (red) and TRX-fused TALE^centro (TTALE^centro) signals. Dashed lines indicate the nuclear boundary. Scale bars, 5 μm. (C) Histograms showing numbers of centromeric FISH- and peptide-fused TALE^centro(19 bp)-positive dots in nuclei of HeLa cells. n = 50 nuclei per group.