Fig. 6.

Influence of bioactive compounds on TRX1 levels, subcellular localization and redox state. LNCaP (A, C) and PC-3 (B, D) cells were treated with 1 mM melatonin, 50 µM silibinin, 25 µM curcumin or 50 µM resveratrol for 48 h and TRX1 protein levels were evaluated by western blot. HDAC2 and β-Actin were employed as loading controls. Redox state of TRX1 in LNCaP (E) and PC-3 (F) was analyzed after 48 h of treatment. Experiments were repeated at least 3 times and data of a representative experiment is shown.