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. 2005 Jan;79(2):1084–1098. doi: 10.1128/JVI.79.2.1084-1098.2005

FIG. 7.

FIG. 7.

EGFP-F1L-K219A and EGFP-F1L-K219/222A colocalize with calnexin. HeLa cells were transfected with either pEGFP-empty vector (a), pEGFP-F1Lwt (e), pEGFP-F1L-K222A (i), pEGFP-F1L-K219A (m), or pEGFP-F1L-K219/222A (q) and stained with anticalnexin, an ER resident protein, to detect the ER (b, f, j, n, and r). Merged images and intensity plots (c, d, g, h, k, and l) indicate that the majority of EGFP, EGFP-F1Lwt, and EGFP-F1L-K222A do not colocalize with calnexin. The merge image of EGPF-F1L-K219A and calnexin (o) indicates partial ER localization, which is further supported by the merge intensity plot (p). The merge image of EGFP-F1L-K219/222A and calnexin (s) indicates EGFP-F1L-K219/222A colocalizes with calnexin, and this is further supported by alignment in the staining intensity plot (t).