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. 2017 Mar 1;7(3):473–483.

Figure 2.

Figure 2

Chk1 inhibitor LY2606368 can induce DNA damage and apoptosis, and can suppress cell proliferation in gastric cancer cells. A. Graphical presentation of % cell viability of AGS and MKN1 cells measured 3 days after treatment with LY2606368. B. Clonogenic assay in AGS and MKN1 cells. Cells were treated with LY2606368 for 3 days. Cell viability in AGS and MKN1 cells were significantly inhibited in a dosage-dependent manner. C. Graphical presentation of relative (%) colony formation of AGS and MKN1 cells in clonogenic assay as described in B after exposure to 25 nM LY2606368 for 24 hours. D. Graphical presentation of apoptosis in AGS and MKN1 cells, measured using Annexin V/PI after exposure to 25 nM LY2606368 for 24 hours. Significant apoptosis was observed in AGS and MKN1 cells staining. E, F. Western blot analyses of AGS and MKN1 cells treated with LY2606368 for 24 hours. Endogenous Chk1, γ-H2AX, cleaved caspase3, and p-Chk1 (Ser345) were detected using their respective antibodies as shown left to each panel.