Figure 3.

Chk1 inhibitor LY2606368 can suppress HR repair capacity. DR-GFP cells were used to detect the effect of LY2606368 on HR repair. A. LY2606368 treatment (20 nM) for 24 hours suppresses the HR repair capacity (P<0.05, LY20606368 VS DMSO). Each value was presented with the percentage of the GFP positive cells in pCBASecI transfected cells. The fold change was calculated, and data was showed as Mean ± SD. B. Cell cycle in HR repair assay was analyzed. LY2606368 induces S phase arrest in DR-GFP cells. C. HU was used to synchronize the cell cycle distribution for 16 hours before HR repair analysis, fold change was showed as mean ± SD. D. Cell cycle analysis for HU-synchronized HR repair assays showed there was no significant cell cycle alternation between DMSO control and LY2606368 treated groups after HU synchronization.