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. 2005 Jan;79(2):1133–1141. doi: 10.1128/JVI.79.2.1133-1141.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 5. — HIV-1 Gag and Hck form stable complexes with NMT isoforms. Detergent extracts prepared from Cos7 cells transiently coexpressing T7 epitope-tagged NMT-1 and NMT-2 alone (lane 1) or together with H epitope-tagged HIV-1 Gag (Gag.H) (lane 3), Hck (Hck.H) (lane 4), or, as controls, wild-type NA7 (NA7.AH) (lane 2) or myristoylation-defective [NA7_(G2A).AH] (lane 5) HIV-1 Nef were immunoprecipitated with α-H MAb (IP:α-H), and immunoprecipitates were resolved by SDS-PAGE and analyzed by Western blotting as described in the legend to Fig. 3.