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. 2017 Mar 1;7(3):531–542.

Figure 4.

Figure 4

Deletion of integrin β1 expression inhibits the motility of ESCC cells by suppressing FAK-Rac1 signaling. A: siRNA knockdown of FAK expression significantly reduced the FAK level in 30D cells or TE10 cells. B: Deletion of FAK in 30D or TE10 cells impeded their motility in vitro relative to the control cells. In the Transwell assays, five fields were randomly selected and the invading cells were counted. All scale bars in these images indicate 100 μm. C: In the stably β1-deleted 30D cells (30D-shITGB1-3/7), the levels of phosphorylated FAK (Y397/576/577/861/925), P130/Cas (Y16/249/410), and paxillin (Y118) were reduced compared with control cells (30D-shGFP) under adherent or chemotaxis conditions (2 h). D: Under chemotaxis conditions (2 h), 30D cells from the shITGB1-3/7 group expressed lower levels of Rac1-GTP than shGFP cells. E: Stable deletion of integrin β1 in TE10 cells (TE10-shITGB1-3/7) also reduced the level of phosphorylated FAK (Y397/576/577/861/925), P130/Cas (Y16/249/410), and paxillin (Y118) relative to the control ones (TE10-shGFP) under chemotaxis conditions for 2 h.