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ACSM3 has no effect on fatty acid metabolism. A: Oil Red O staining showed the neutral lipid content of cells. Nucleus was counterstained with DAPI. B: Quantitative analysis of Oil Red O staining by measuring the absorbance (OD at 490 nm) of extracted dye. C: Quantitative real-time PCR analysis for expression of FASN, SCD, CPT1A and ACOX1 in SMMC7721 and HepG2 cells with ACSM3 overexpression or control.
