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. 2017 Mar 1;7(3):673–687.

Figure 6.

Figure 6

Rough eye phenotypes are enhanced in proportion to the number of copies of UAS-S1-d but not UAS-FL. A-D. In homozygous FL expressing flies, eye morphology is the same as in heterozygotes. In contrast, the rough eye phenotype in S1-d heterozygotes is enhanced in heteroallelic combinations. A-D. Each panel shows a scanning electron micrograph of an adult compound eye. A-D. Scale bar, 66.6 μm. A. GMR-GAL4/+; UAS-GFP/+; UAS-yki FL (8-1-1)/+, B. GMR-GAL4/+; +; UAS-FL (8-1-1), C. GMR-GAL4/+; UAS-GFP/+; UAS-S1-d (18-3-1)/+, D. GMR-GAL4/+; +; UAS-S1-d (18-3-1)/(20-3-1), E. Relative expression levels of yki mRNA normalised to GAPDH mRNA. The relative expression levels of yki mRNA in flies carrying 1 copy and 2 copies of UAS-S1-d are not significantly different. F. Yki protein is significantly increased in fly carrying 2 copies of UAS-S1-d, yielding similar levels of protein as produced by fly carrying 1 copy of UAS-ΔSL2. Data are means SD (n = 3). *P<0.05, **P<0.01 (one-way ANOVA, Dunnetts Multiple comparisons test).