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. Author manuscript; available in PMC: 2017 Apr 10.
Published in final edited form as: Biochim Biophys Acta. 2017 Jan 8;1860(4):438–449. doi: 10.1016/j.bbagrm.2017.01.003

Figure 6. Chromatin states mark the dynamic activities of cis-regulatory modules during MSC commitment to osteoblast.

Figure 6

A) Chromatin states indicate dynamic enhancer regions flanking the Fgfr1 gene. Genomic regions identified as osteogenesis specific (gaining H3K27ac during differentiation) are boxed in blue and H3K27ac and constitutive enhancers boxed in red and include annotations for VISTA-identified enhancers that demonstrate tissue-specific expression of beta-galactosidase during embryogenesis [13]. B) p300 occupancy at mm610 and mm612 regions was assessed by ChIP and is consistent with enrichment of H3K27ac at these regions during osteoblast differentiation. * Significant different (p ≤ 0.05) by t-test. C) Relative expression of genes flanking the 1 Mb regions of Fgfr1 relative to day 0. * Significantly different (p ≤ 0.05) by two-way ANOVA. D) Chromatin states on chromosome 5 at a gene cluster heavily involved in osteogenesis. Chromatin states in the constitutive active Spp1 and osteogenesis-upregulated Dmp1 were are highlighted (in blue) and demonstrate constitutive and dynamic enhancer acquisition, respectively.