FIG. 6.

Budding capsids at Golgi membranes and formation of transport vacuoles (wrapping) at advanced and late stages of infection, at an MOI of 1 (F), 5 (B, C, and E), or 10 (D and G). (A) Capsid approaching a Golgi membrane close to two dilated nuclear pores (np). (B) Capsid less than 100 nm from the outer nuclear membrane (o), in initial phase of budding with slight bending and thickening of the membrane in contact with the capsid. (C) Intermediate phase of budding with thickening of the membrane and little deposition of tegument. cp, coated pit. (D) Late phase of budding with tegument surrounding the capsid, clearly visible spikes, and start of fusion (fu) of Golgi membranes at the basal pole; wrapping is initiated by initation of fission (fi; right) but is close to completion at the left (fi; arrow) forming a spherical vacuole containing the virion (tangentially sectioned) and a dense substance completely hiding the spikes. (E) Spherical transport vacuole comprising a virion and dense substance-hiding spikes. (F) The membrane of a transport vacuole with a tangentially sectioned virion fuses with the plasma membrane (pm) releasing virion and antifusion proteins into the extracellular space. (G) Virion in the extra cellular space, comprising capsid with dense core, tegument, and envelope with spikes. Bars, 100 nm.