FIG. 7.

Spread of rgPIV3 infection is compromised in HAE derived from CF airway epithelium. rgPIV3-mediated expression of GFP viewed en face in HAE derived from a non-CF patient (A, C, E, and G) and from a CF patient (B, D, F, and H). Inoculation of the apical surfaces of non-CF (A) or CF (B) HAE with a high titer of rgPIV3 (10⁶ PFU) resulted in similar levels of infection at 24 h p.i. Similarly, with a low-titer rgPIV3 inoculum (10³ PFU), lower but comparable numbers of GFP-positive cells were detected 24 h after inoculation in non-CF (C) and CF (D) HAE. Following the pattern of viral spread with time revealed that viral spread was facilitated by coordinated cilial beat in non-CF HAE by 48 h p.i. (E), leading to homogenous distribution of infection by 72 h (G). In contrast, for CF HAE, viral spread was restricted to cells in close proximity to the primary infected cells and spread remained limited at both 48 (F) and 72 (H) h p.i. Original magnification, ×10.