FIG. 8.

rgPIV3-induced ciliated cell shedding leads to mucin-containing cell metaplasia in HAE. (A) Quantitation of the percentage of GFP-positive cells over time after apical inoculation of HAE with rgPIV3 (10⁶ PFU; closed triangles) or AdVGFP (10¹⁰ p/ml; inoculated after sodium caprate treatment to open epithelial tight junctions; closed circles). (B) Transepithelial resistance measurements over time after inoculation of HAE with rgPIV3 (open bars) or vehicle control only (filled bars). (C) Representative histological sections of perfluorocarbon-osmium tetroxide-fixed HAE inoculated with either vehicle control (i and iii) or rgPIV3 (ii and iv) and fixed 2 days p.i. (i and ii) or 13 days p.i. (iii and iv). Note the increased presence of mucin-containing cells (indicated by arrows) in HAE inoculated with rgPIV3 and fixed 13 days p.i. Bar, 20 μm.