Figure 2. Overexpression of miR-198 inhibits colorectal cancer cell proliferation, migration and invasion in vitro.

SW1116 and HCT116 cells were stably transfected with a miR-198 overexpression plasmid or control negative plasmid for subsequent analysis. Data in all statistical plots represent means ± SD. (A,B) Proliferation curves of SW1116 and HCT116 cells, respectively, as determined by CCK-8 assay. The rate of proliferation of the miR-198 overexpression cell lines was significantly inhibited compared with that of the control cells (P < 0.001, two-sided t-test). (C,D,E,F) Cell migration (at 24 h and 36 h) and invasion ability (at 48 h and 60 h) in SW1116 (C,D) and HCT116 (E,F) cells were analyzed in transwell assays. Migrated cells and invaded cells stained by 0.1% crystal violet were counted in 8 randomly microscope fields (×200). Statistical analyses are based on three independent experiments and representative images are shown. MiR-198 overexpression reduced the migration and invasion ability of both cell lines compared with the negative controls. (G, H) Wound healing assays demonstrated that miR-198 expression significantly reduced the proliferation and migration of SW1116 and HCT116 cells (P < 0.01). Statistical analysis is based on three independent experiments and representative images are shown.