FIG. 5.

JSRV Env protein does not coimmunoprecipitate with PI3K (p85) in the transformed MDCK cells. Preparation of samples was the same as described in the legend to Fig. 4. The resulting immunoprecipitates and cell lysates were subjected to SDS-PAGE, immunoblotted with anti-p85 (A), and then stripped and reblotted with anti-FLAG (B). IgG H and IgG L indicate the IgG heavy and light chains, respectively.