FIG. 1.

Protein composition of gradient-purified KSHV virions. Purified virion lysate was resolved on 3 to 8% Tris-acetate (lane 2) and 12% Bis-Tris NuPAGE (lane 4) gels with molecular markers (lanes 1 and 3) and stained with colloidal Coomassie G-250. Prominent bands were excised, digested in gel with trypsin, and subjected to LC-MS/MS analysis. The resultant MS/MS spectra were run against a sequence database with the SEQUEST program. Matched KSHV or cellular proteins are indicated at the right of each gel.