FIG. 5.

Effect of trypsin digestion and detergent treatment on association of cellular cytoskeletal proteins with purified KSHV virions. Purified virions were treated with trypsin either in the absence (lane 1) or in the presence (lane 2) of 1% Triton X-100 for 1 h at 37°C or left untreated (lane 3). The proteolysis reactions were terminated by addition of 0.5 mM PMSF and 1/100 volume of protease inhibitor. The samples were analyzed by Western blotting with antibodies against β-actin and class II myosin heavy peptide A, respectively. In addition, virions were left untreated (lane 4) or treated with 1% Triton X-100 plus 0.5% DOC for 30 min and then centrifuged at 100,000 × g for 1 h. The supernatant (Sup) (lane 5) and pelleted tegument-capsid (lane 6) were analyzed by Western blotting. The values on the left are molecular sizes in kilodaltons.