Figure 2. Sevoflurane decreased the viability of rat FNSCs in a dose- and time-dependent manner.

Data were obtained from at least four separate cultures, presented as mean ± standard deviation, and analyzed by two-way ANOVA followed by Bonferroni’s multiple comparison tests (n ≥ 6 for each condition). *P < 0.05 or ***P < 0.001, respectively, compared with controls. Exposure of FNSCs to 1.2% sevoflurane for 6 h, 12 h, and 24 h had no effect on survival, but exposure to 2.4% sevoflurane, a clinically relevant concentration, resulted in significant cell damage at 24 h (***P < 0.001), as measured by the MTT assay. Exposure to 4.8% sevoflurane for 6 h could decrease cell viability and more significantly for 12 h and 24 h (***P < 0.001).