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. 2017 Apr 10;7:46304. doi: 10.1038/srep46304

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Copyright © 2017, The Author(s)

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(a) Immunofluorescence images were obtained using SOX2 (pink) staining 24 h later and DAPI (blue) nuclear staining (undifferentiated neural stem cells), and the images were merged. (b) Sox2 immunofluorescence images from FNSCs exposed to 4.8% sevoflurane for 6 h. (c) Statistical results of SOX2-positive cell ratio in FNSCs exposed to different concentrations of sevoflurane (1.2%, 2.4%, and 4.8%) for 6 h. Data were obtained from at least three separate cultures, ***P < 0.001 versus control group.