| Tilt series settings | |
|---|---|
| Tilt angle range | ±60–65° |
| Tilt increment | 1–2° |
| Scheme | Bidirectional from 0° |
| Beam intensity | Constant |
| Exposure time | 1/cosine1/5 weighted |
| Defocus | −4–6 μm |
| Additional focus offset | 2–3 μm |
| Detector frame rate | 12–24 f/p/s |
| Total dose | 60–150 e−/Å2 |
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