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. Author manuscript; available in PMC: 2018 Feb 17.
Published in final edited form as: ACS Chem Biol. 2016 Dec 22;12(2):407–413. doi: 10.1021/acschembio.6b00981

Figure 5.

Figure 5

Imaging membrane potential changes in cultured rat hippocampal neurons and hPSC-derived midbrain dopaminergic (mDA) neurons using dsVF2.2(OMe).Cl. a) Confocal images of rat hippocampal neurons stained with 500 nM dsVF2.2(OMe).Cl. * and ** are zoomed regions of the indicated neurons b) Transmitted light image (DIC) of hPSC-derived mDA neurons. c) Wide field fluorescence image of mDA neurons from panel b stained with 500 nM dsVF2.2(OMe).Cl. d) Fractional change in fluroescence vs. time for cells indicated in panels b. Each trace represents the fluorescence intensity from the indicated cell; all traces have been bleach corrected and are unfiltered. Scale bars are 20 μm.