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. 2017 Apr 1;28(7):922–934. doi: 10.1091/mbc.E16-10-0733

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© 2017 van Haastert et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Ras-GTP patches are not strongly influenced by neighboring patches. (A) Schematic of measurements. Left, a patch has a width w, lifetime t, intensity i, and given times of birth and death. Right, a patch is embedded by adjacent patches and has distances x and y to the adjacent patch to the right and left, respectively; these are measured at the times of birth (x_b and y_b) during life (x_l and y_l) and at the times of death (x_d and y_d) for the patch. (B) Lack of correlation between lifetime, width, or intensity of a patch and the distance to the adjacent patch (n = 23). All R² values are very low and do not support a significant correlation. (C) A cell may have one or multiple Ras-GTP patches and patch-free spaces. The width of a Ras-GTP patch is independent of the number of other patches present; instead, patch-free spaces become smaller when more patches are present. Data are means and SEM from 50 patches. (D) Probability distribution of the appearance of a new Ras-GTP patch relative to the position of the two adjacent patches; at the moment of birth of a new patch, we measured distances x_b and y_b to the adjacent patches (data from 90 experimental Ras-GTP patches and 124 model R patches). The results show that a new Ras-GTP patch frequently starts somewhere in the middle of the open space between two patches. The data include cells with a single existing patch, where x_b and y_b represent the distances of the new patch to the right and left sides of that existing patch; the new patch preferentially appears at the opposite side of the existing patch. See Supplemental Figure S1 for other properties of Ras-GTP patches.