Figure 3.

Trypsin Inhibition by Wild-Type and Mutant S. moellendorffii BBI3.

(A) Full-length wild-type BBI3 showing predicted inhibitory residues (bold).

(B) Partial BBI3 sequences showing mutations (black highlight).

(C) Inhibitors were incubated with trypsin and a colorogenic substrate of trypsin whose activity is measurable as OD₄₁₀. Error bars represent sd for three technical replicates in a single microtiter plate. Trypsin incubated with the colorogenic substrate but no inhibitor was used as a control (NIC).