Fig. 2.

Black ginseng-enriched Chong-Myung-Tang (CMT) extracts suppressed lipopolysaccharide (LPS)-mediated nitric oxide (NO) release without showing cytotoxicity. (A) BV2 cells were seeded in 24-well plates overnight and then preincubated with CMT extracts (250–1,000 μg/mL) for 30 min and stimulated with LPS (0.1 μg/mL) for 18 h. The cell supernatant was then transferred to 96-well plates and homogenized with equal amounts of Griess reagent, and NO production was measured at 540 nm. (B) Effects of CMT extracts on cell viability determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and absorbance measurement at 560 nm (B). Values in bar graph are mean ± standard deviation of at least three independent experiments. *p < 0.005 and **p < 0.001 compared with LPS only.