Fig. 3.

Black ginseng-enriched Chong-Myung-Tang (CMT) extracts inhibited messenger RNA (mRNA) expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α) in BV2 cells. (A and B) BV2 cells were pretreated with black ginseng-enriched CMT extracts (250–1,000 μg/mL) for 30 min and then stimulated with lipopolysaccharide (LPS; 0.1 μg/mL) overnight. Total RNA was extracted by TRIzol RNA extraction reagent and mRNA expression of iNOS, COX-2, IL-1β, IL-6, and TNF-α was determined by reverse transcription-polymerase chain reaction. GAPDH was used as the housekeeping gene. Images are representative of three independent experiments. Values in bar graph are mean ± standard deviation of four independent experiments (B). *p < 0.01, **p < 0.005, and ***p < 0.001 compared with LPS only. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.