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. 2016 Mar 25;41(2):151–158. doi: 10.1016/j.jgr.2016.02.004

Fig. 5.

Fig. 5

Diminution of nuclear factor-κB (NF-κB) pathway by black ginseng-enriched Chong-Myung-Tang (CMT) extracts. BV2 cells were pretreated with CMT extracts and then stimulated with or without lipopolysaccharide (LPS; 0.1 μg/mL) overnight. Nuclear and cytosolic proteins were separated using the NE-PER extraction kit. Data are mean ± standard deviation (n = 3); *p < 0.01, **p < 0.005, ***p < 0.001 compared with LPS only. (A) Phosphorylation of transforming growth factor beta-activated kinase 1 (P-TAK-1) compared with total (T-TAK1). (B) Phosphorylation of IκB kinase (P-IKK) α/β compared with β-actin. (C) Phosphorylation of P-IκBα compared with total IκBα. (D) Phosphorylation of NF-κB compared with β-actin.