Table 1.
Gene | Primer | oligonucleotide sequence (5′→3′) |
---|---|---|
GAPDH | F | 5′-CAATGAATACGGCTACAGCAAC-3′ |
R | 5′-AGGGAGATGCTCAGTGTTGG-3′ | |
iNOS | F | 5′-CCCTTCCGAAGTTTCTGGCAGCAGC-3′ |
R | 5′-GGCTGTCAGAGCCTCGTGGCTTTGG-3′ | |
COX-2 | F | 5′-TCTCAGCACCCACCCGCTCA-3′ |
R | 5′-GCCCCGTAGACCCTGCTCGA-3′ | |
IL-1β | F | 5′-CAGGGTGGGTGTGCCGTCTTTC-3′ |
R | 5′-TGCTTCCAAACCTTTGACCTGGGC-3′ | |
TNF-ɑ | F | 5′-TTGACCTCAGCGCTGAGTTG-3′ |
R | 5′-CCTGTAGCCCACGTCGTAGC-3′ | |
IL-6 | F | 5′-GTACTCCAGAAGACCAGAGG-3′ |
R | 5′-TGCTGGTGACAACCACGGCC-3′ |
COX-2, cyclooxygenase-2; F, forward; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL, interleukin; iNOS, inducible nitric oxide synthase; R, reverse; TNF-α, tumor necrosis factor-α