Fig. 7.
Effect of Korean Red Ginseng extract (RGE) on the mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) signaling pathway in HMC-1 cells. Phosphorylation of MAPKs (A, B) and the degradation of IκBα and translocation of NF-κB (C, D) were assayed by a Western blot analysis. HMC-1 cells (1 × 106 cells/well) were incubated with various concentrations of RGE and then stimulated with phorbol 12-myristate 13-acetate (PMA; 50 nM) + A23187 (1 μM) for 30 min (A, B) or 2 h (C, D). C, cytosol extracts; N, nuclear extracts. (B, D) Densitometry analyses presented as relative ratios of total protein (B) and housekeeping protein (D) were used as a loading control. The data shown are representative of three independent experiments. Values represent the mean ± standard deviation (SD) of three independent experiments. *p < 0.05 versus blank and **p < 0.05 versus PMA + A23187 alone.