Table 1.
Sequences of the Real-Time reverse Transcription Polymerase Chain Reaction (RT-PCR) primers
| Genes | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|
| IL-8 (m) | TGTAATGAAAGACGGCACACC | TCTTCTTTGGGTATTGCTTGG |
| MDC (m) | CCAAGGTGCCTTTGAAGACT | TCCTCCAGCTGGTGGTTACT |
| TARC (m) | CCAGGAAATCTCTGGTCTGC | CTGTGGAAGCAGTGGAGTCA |
| GAPDH (m) | GACATGCCGCCTGGAGAAAC | AGCCCAGGATGCCCTTTAGT |
| IL-8 (h) | AAGCTGGCCGTGGCTCTCTTG | AGCCCTCTTCAAAAACTTCTC |
| TNF-α (h) | CGCTCCCCAAGAAGACAG | AGAGGCTGAGGAACAAGCAC |
| MDC (h) | AGGACAGAGCATGGATCGCCTACAGA | TAATGGCAGGGAGGTAGGGCTCCTGA |
| TARC (h) | CTTCTCTGCAGCACATCC | AAGACCTCTCAAGGCTTTG |
| MIP-1α (h) | GTCTGTGCTGATCCCAGTGA | TTGTCACCAGACGCGGTGTG |
| MIP-1β (h) | GTCTGTGCTGATCCCAGTGA | GGACACTTATCCTTTGGCTA |
| RANTES (h) | CCGCGGCAGCCCTCGCTGTCATCC | CATCTCCAAAGAGTTGATGTACTCC |
| MCP-1 (h) | GTCTCTGCCGCCCTTCTGT | TTGCATCTGATGGCAGTAGCT |
| GAPDH (h) | TGCACCACCACCTGCTTAGC | GGCATGGACTGTGGTCATGAG |
GAPDH, glyceraldehyde 3-phosphate dehydrogenase; h, human; IL-8, interleukin-8; m, mouse; MCP-1, monocyte chemoattractant protein-1; MDC, macrophage-derived chemokine; RANTES, regulated on activation, normal T cell expressed and secreted; TARC, thymus and activation-regulated chemokine; TNF-α, tumor necrosis factor-α.