Table 1.

Oligonucleotide primers used for PCR amplification reactions.

Oligonucleotide	Sequence (5′ → 3′)a
His_SlpB_NcoI_for	ctgctgCCATGGGCCACCACCACCACCACCATAAATCATATGCCAAAGTTACATC
SlpB_NheI_for	aatcaGCTAGCAAATCATATGCCAAAGTTACATC
SlpB_XhoI_rev	ctgctgCTCGAGTTAATTAAACGGTGTAACAGTAACAG
SlpB_His_XhoI_rev	aatcaCTCGAGTTAATGATGATGATGATGATGGCTGCTGCCGCTGCCGCGCGGCACCAGCGCATTAAACGGTGTAACAGTAAC
SlpB_AH3a42_1_rev	CTGCATTAAATGCTGTTCGCACGGGCGTAAGTTCGCGCGTTCTAACTGGCTCTGGCAGCGGCGATTAAACGGTGTAACAGTAAC
SlpB_AH3a42_2_XhoI_rev	aatcaCTCGAGTTA ATACGGATCCTGGCTCGGGCTATACGGATCGCGGCCATAGCTATCTTCATCGCGCTGAATTTTCTGCATTAAATGCTGTTCG

^aArtificial restriction sites are underlined. Lowercase letters indicate artificially introduced nucleotides to improve restriction enzyme cutting. The AH3a-42 gene is indicated in bold italic.