Table 1.
Oligonucleotide | Sequence (5′ → 3′)a |
---|---|
His_SlpB_NcoI_for | ctgctgCCATGGGCCACCACCACCACCACCATAAATCATATGCCAAAGTTACATC |
SlpB_NheI_for | aatcaGCTAGCAAATCATATGCCAAAGTTACATC |
SlpB_XhoI_rev | ctgctgCTCGAGTTAATTAAACGGTGTAACAGTAACAG |
SlpB_His_XhoI_rev | aatcaCTCGAGTTAATGATGATGATGATGATGGCTGCTGCCGCTGCCGCGCGGCACCAGCGCATTAAACGGTGTAACAGTAAC |
SlpB_AH3a42_1_rev | CTGCATTAAATGCTGTTCGCACGGGCGTAAGTTCGCGCGTTCTAACTGGCTCTGGCAGCGGCGATTAAACGGTGTAACAGTAAC |
SlpB_AH3a42_2_XhoI_rev | aatcaCTCGAGTTA ATACGGATCCTGGCTCGGGCTATACGGATCGCGGCCATAGCTATCTTCATCGCGCTGAATTTTCTGCATTAAATGCTGTTCG |
Artificial restriction sites are underlined. Lowercase letters indicate artificially introduced nucleotides to improve restriction enzyme cutting. The AH3a-42 gene is indicated in bold italic.