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. 2015 May 11;5:10152. doi: 10.1038/srep10152

Figure 10. Schematic representation of the pCAGGS-rtTA-TRE-GH12 (pTTGH) vector fragment.

Figure 10

A) Schematic of the pTTGH vector. B) pCAGGS-rtTA-loxp-neoTK-TRE-GH12 fragment (pTTGH, Ssp I, Sfi I cut, 8257 bp). The pTTGH vector was cut with restriction enzymes, Ssp I and Sfi I, and the pTTGH fragment (8257 bp) was recovered. The pTTGH fragment was constructed with the GH expression box, a GH12 gene controlled with a pTRE promoter, a GH expression inducing box and an rtTA gene expressed with a pCAG promoter. A fusion gene of a neomycin resistant gene and a diphtherial toxin (DTA) gene, flanked by loxP sites, was chosen as a selectable gene. The relative positions of the primer pairs pGH-SL/ pGH-SR, pGH-L/ pGH-R, rtTA-L/rtTA-R, rtTA-L2/rtTA-R2 and 3 sequence specific primers for genome walking are shown. The EcoRI restriction enzyme sites (444 bp, 928 bp, 4204 bp and 7232 bp) are shown above.