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. 2015 May 11;5:10152. doi: 10.1038/srep10152

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Copyright © 2015, Macmillan Publishers Limited

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A) Detection of the rtTA gene in genomic DNA from blood samples of the 5 cloned pigs (lanes 4–8) and a non-transgenic pig (lane 2). Lane PC, using a pTTGH DNA fragment as a template; lane NC, using genomic DNA from non-transgenic pigs as a template. B) Transgenic pigs were identified using Southern blot and a Dig-labeled GH probe was used to hybridize the genomic DNA from ear tip tissue of transgenic pigs. After digestion with EcoRI, the endogenous GH (pGH1 in B) band and part of the pTTGH fragment (pGH2 in B) were detected in transgenic pigs. rtTA was also detected. The blots have been cropped to focus on the bands of interest. See Supplementary Fig. S4-5 for full-length gels.