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. 2015 Apr 30;5:9802. doi: 10.1038/srep09802

Figure 2. SMIFH2 affects the cytoskeleton and the Golgi complex of HCT116 cells.

Figure 2

(A) SMIFH2 induces dynamic cytoskeletal remodelling in wild-type HCT116 cells. Wild-type HCT116 cells were treated as in Fig. 1A. Fixed cells were stained for F-actin (TRITC-Phalloidin), β-tubulin (Alexa-488), Giantin (Alexa-647; red in merge) and the nucleus (DAPI; blue in merge). Representative maximal confocal projections are shown. Scale bar, 10 µm. (B) SMIFH2 decreases p53 levels in HCT116 cells. Wild-type HCT116 cells were treated with SMIFH2 or DMSO for the indicated time (Time, (h) = hour). Total cell lysates were separated by SDS-PAGE and blotted with the indicated antibodies. Vinculin served as loading control. Gels were run under the same experimental conditions and blots were cropped for final display.