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. 2015 May 21;5:10482. doi: 10.1038/srep10482

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Effects of BLG knockout in goat’s milk. (A) Analysis of milk from mono-allelic BLG-targeted goats. (top) Analysis of whey proteins by Coomassie blue staining after separation by SDS/PAGE. Equal amounts of milk samples were loaded onto each lane of the gel. (bottom) Western blot analysis of hLF expression. (B) Analysis of BLG expression through qPCR. Values with different superscripts are significantly different (P < 0.05). Three assay replicates were performed for each milk sample. (C) Analysis of milk from bi-allelic BLG-targeted goats. (top) Analysis of whey proteins by Coomassie blue staining after separation by SDS/PAGE. Equal amounts of milk samples were loaded onto each lane of the gel. (bottom) Western blot analysis of hLF expression. (D) Reverse-transcription PCR analysis of BLG mRNAs in cells from the milk of bi-allelic BLG-targeted goats.