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. 2017 Apr 10;12(4):e0175326. doi: 10.1371/journal.pone.0175326

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Fig 1 — B. bronchiseptica (B.bronchi), B. holmesii, and B. parapertussis (B.para) isolates (n = 14) were used to evaluate assay specificity, alongside B. pertussis Pt-negative isolates, I979 and J365. The H921 isolate is the B. pertussis Pt-positive isolate that was used as the positive control. Phosphate-buffered saline (PBS) was used as an additional assay control to screen for cross-reactivity. RLU = relative light units.