Table 1. Variability between runs*.
| Isolate | Mean FOC | %CV |
|---|---|---|
| I125 | 1.034 | 7.59 |
| I149 | 1.118 | 10.47 |
| I315 | 1.087 | 9.30 |
| I327 | 1.121 | 12.74 |
| I337 | 1.015 | 10.10 |
| I338 | 0.828 | 10.00 |
| I339 | 0.817 | 14.71 |
| I340 | 0.817 | 14.71 |
| I342 | 1.027 | 9.82 |
| I343 | 1.056 | 11.45 |
| I344 | 0.766 | 12.85 |
| I347 | 1.226 | 10.51 |
| I348 | 1.046 | 10.63 |
| I359 | 1.107 | 12.27 |
| I430 | 1.012 | 12.36 |
| I434 | 1.111 | 11.05 |
| I437 | 1.227 | 9.72 |
| I746 | 0.751 | 14.57 |
| I747 | 0.956 | 13.94 |
| I748 | 1.005 | 11.66 |
*Assay variability between runs was evaluated on 20 B. pertussis isolates. Isolates were prepared as described above and stored at 4°C for the duration of the study. Each prepared isolate was tested on 5 different days, with 10 replicates per plate. Four replicates of positive control isolate (H921) was included on each plate. To normalize results for each isolate we averaged signal values for positive control on each plate and expressed isolate signal values as a fraction of a positive control signal. FOC = fraction of control.